PANELS CUSTOMISES NGS
CONSTITUTIONNEL - HEMATOLOGIE - ONCOLOGIE



KITS ENRICHISSEMENT NGS
LOGICIEL INTERPRETATION/ANNOTATION






 

PANELS NGS A FAÇON


Couverture & Uniformité >
    Performance clinique validée
ADN circulant, FFPE 
Kits compatibles avec tout type de plate-forme NGS
Illumina, Ion Torrent, MGIseq
ISO 9001 - ISO 13485 - GMP

Customized Kit :
Library Preparation Kit + Adaptors and Index Primers Set + Customized Target capture reagent (Customized Probe, Hyb. Reagent and Wash Buffers)

Etapes Description

Customized Panels enable you to design target size up to a maximum of 1.5 Mb

Target Region Option CDS, CDS+UTR, Intron ...


Advance your customized panel design
Upon your request, we can design and optimize panels to detect SNV, InDel, CNV, and Gene Rearrangement mutations through utilizing our probe rebalancing technology.

Check Pilot Test Performance
Pilot test results from customized kits can be produced and shipped within 4-6 weeks from the date requested.

Make Your Business Scalable
Scalable system (custom-made kits) production in large volume is available.


 

KITS NGS GENES CIBLES


Description du Kit Régions / Gènes ciblés
Contenu du kit
BRCA1 & BRCA2

CE/IVD

Whole CDS (+/- 40 bp) region of BRCA1 & BRCA2 (Target size: 17kb)

SNV, Indel, CNV

Exon (Exon +/- 40 bp)

Library Preparation kit + Adaptors and Index Primers set + BRCA 1,2 Target capture reagent

(Probe, Hyb. Reagent and Wash Buffers)

OncoRisk
(31 gènes)

SNV, Indel, CNV

Whole CDS (+/- 40 bp) region (Target size: 97kb)

APC, ATM, BARD1, BLM, BMPR1A, BRCA1, BRCA2, BRIP1, CDH1, CDK4, CDKN2A, CHEK2, EPCAM, MLH1, MRE11A, MSH2, MSH6, MUTYH, NBN, PALB2, PMS2, PRSS1, PTEN, RAD50, RAD51C, RAD51D, SLX4, SMAD4, STK11, TP53, VHL

Library Preparation kit + Adaptors and Index Primers set + OncoRisk Target capture reagent

(Probe, Hyb. Reagent and Wash Buffers)

CancerScreen
(SNV, Indel, CNV)
(15 gènes)

CDS + Intron(Gene fusions)

Rearrangement: ALK, RET, ROS1
Amplification : ERBB2, MET
SNV, Indel: APC, BRAF, EGFR, ERBB2, KRAS, MET, NRAS, PIK3CA, SMAD4, TP53

Library Preparation kit + Adaptors and Index Primers set + CancerScreen Target capture reagent

(Probe, Hyb. Reagent and Wash Buffers)

PharmacoScreen

Target size: 132Kb, Target genes: 20 genes, 31 Drug lists

Library Preparation kit + Adaptors and Index Primers set + PharmacoScreen Target capture reagent

(Probe, Hyb. Reagent and Wash Buffers)


PRODUCT COMPOSITION

Sample Library Preparation

End repair enzyme mix / A-tailing enzyme mix / Ligation enzyme mix

Hybridization

Target specific hybridization probe Hybridization Buffers and Blocking mix

Index Kit

16rxn package includes 16 index (Single index type)

48rxn package includes 48 index (Dual index type)

96rxn package includes 96 index (Dual index type)

Post Hybridization Wash

Buffers for post hybridization wash

SAMPLE QUALITY RECOMMENDATION

Human genomic DNA : blood, tissue, saliva ≥750ng ; FFPE ≥200ng

TEST RECOMMENDATION

Read Length 75 ~ 150 bp (recommended, but depending on sequencer type)

Supplement

Plastic wares

Magnetic beads for DNA purification (Example: Ampure XP magnetic bead)

Streptavidin coated magnetic bead (Example: Dynabead T1)

Polymerase for amplification  (Example: Kapa Library Amplification kit, Herculase II, 􀀀Phusion Polymerase)

Equipment

DNA shearing device (Example: Covaris)

DNA quantification device (Example: Bioanalyzer, LabChip GX, Tapestation, Qubit)

Thermocycler (PCR machine)

Magnetic Rack

Speed vaccum system

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